SSR PRIMER DERIVED FROM ACTINIDIA ARGUTA AND USE THEREOF

摘要

PURPOSE: A Actinidia arguta (Siebold & Zucc.) Planch. ex Miq. var. arguta-isolated SSR primer is provided to detect DNA polymorphism and to distinguish sex and species of Actinidia arguta (Siebold & Zucc.) Planch. ex Miq. var. arguta. CONSTITUTION: An SSR(simple sequence repeat) primer pair contains two nucleotides selected from sequence numbers 1-68. The primer pair is derived from Actinidia arguta (Siebold & Zucc.) Planch. ex Miq. var. arguta. A method for detecting the DNA polymorphism comprises: a step of isolating genome DNA from Actinidia arguta (Siebold & Zucc.) Planch. ex Miq. var. arguta; a step of performing PCR; and a step of separating PCR product by size.