发明名称 |
A DNA NICKING ENZYME FROM A HOMING ENDONUCLEASE THAT STIMULATES SITE-SPECIFIC GENE CONVERSION |
摘要 |
An engineered highly specific DNA-cleavage enzyme delivers a site-specific nick in a double stranded DNA, to cleave one DNA strand within its target site while leaving the opposing DNA strand intact. The engineered enzyme provides the ability to induce a gene conversion event in a mammalian cell. An engineered sequence-specific nickase derived from a LAGLIDADG homing endonuclease is altered by a single amino acid residue, wherein the amino acid residue is involved in the polarization of solvent molecules and acid-base catalysis in the active site without affecting direct contacts between the enzyme and either the bound DNA or bound metal ions. Engineered, site-specific nickase variants, such as of I-AniI and other homing endonucleases, are particularly useful in targeted genome engineering as well as therapeutic, targeted gene repair. |
申请公布号 |
WO2010093966(A3) |
申请公布日期 |
2010.10.14 |
申请号 |
WO2010US24153 |
申请日期 |
2010.02.12 |
申请人 |
FRED HUTCHINSON CANCER RESEARCH CENTER;MCCONNELL-SMITH, AUDREY;STODDARD, BARRY, L. |
发明人 |
MCCONNELL-SMITH, AUDREY;STODDARD, BARRY, L. |
分类号 |
C12N9/22 |
主分类号 |
C12N9/22 |
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