发明名称 |
COMPOSITIONS AND METHODS FOR REGULATING SAS1R |
摘要 |
The present invention provides compositions and methods useful for regulating fertilization and for use as a contraceptive based on the discovery herein of an oocyte specific protein, SAS1R (Sperm Acrosomal SLLP1 Receptor), which is a sperm protein receptor. Six SAS1R variants, including the full length SAS1R, were identified. mSLLP1 and SAS1R co-localized to oocytes and to acrosomes of acrosome-reacted sperm. Interactions between mSLLP1 and SAS1R were demonstrated by far-western analysis, in a yeast two-hybrid system under stringent selection conditions, and by immunoprecipitation of SAS1R by anti-mSLLP1 as well as the converse. Purified recombinant SAS1R was found to have protease activity, to inhibit fertilization in-vitro, and to induce an immune response in females. Together, the results suggest SAS1R is a proteolytically active, oocyte and early embryo specific oolemmal metalloprotease receptor for the sperm intra-acrosomal ligand SLLP1 and is a target for regulating fertilization and as a contraceptive. |
申请公布号 |
WO2010054187(A3) |
申请公布日期 |
2010.10.14 |
申请号 |
WO2009US63540 |
申请日期 |
2009.11.06 |
申请人 |
UNIVERSITY OF VIRGINIA PATENT FOUNDATION;HERR, JOHN C.;SACHDEV, MONIKA;MANDAL, ARABINDA |
发明人 |
HERR, JOHN C.;SACHDEV, MONIKA;MANDAL, ARABINDA |
分类号 |
C12N5/075;A61K38/17;C07K14/705;C12N5/02 |
主分类号 |
C12N5/075 |
代理机构 |
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代理人 |
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