发明名称 |
METHOD FOR PRODUCTION OF SEPARATION MEDIA |
摘要 |
The present invention relates to a method for production of separation media using a so called Spinning Disc technology wherein the porosities of the beads are optimized in such a way that a desired biomolecule may be separated from a complex sample. The method comprises the following steps: a) feeding a 4-8% polysaccharide solution, which has a viscosity within 350-450 mPas, at 65-75° C. to one or more spinning discs at 3001-3010 rpm to form polysaccharide beads; b) capturing said formed polysaccharide beads in a capturing bath; wherein the porosity of the polysaccharide beads is controlled by varying the temperature of the capturing between 15 and 27° C., preferably between 17.5 and 24.6° C. The method yields porosities that prevent molecules larger than 150 000 g/mol to diffuse into the beads. The invention also relates to separation media produced by the method and use thereof for purification of biomolecules, in particular monoclonal antibodies. |
申请公布号 |
EP2237879(A1) |
申请公布日期 |
2010.10.13 |
申请号 |
EP20090707569 |
申请日期 |
2009.01.30 |
申请人 |
GE HEALTHCARE BIO-SCIENCES AB |
发明人 |
GLAD, GUNNAR;JOHANSSON, BO-LENNART;MALOISEL, JEAN-LUC;NORRMAN, NILS;STENHOLM, AAKE |
分类号 |
B01J20/285;B01J2/14;B01J20/26;B01J20/28;B01J20/286;B01J20/287;B01J20/288;B01J20/291;B01J20/32;B01J39/26;B01J41/20 |
主分类号 |
B01J20/285 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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