RECOMBINANT PLASMID pD5spGBD, Escherichia coli STRAIN - RECOMBINANT PROTEIN D5-GBD PRODUCER, RECOMBINANT PROTEIN D5-GBD AND METHOD FOR MAKING THEREOF, METHOD FOR STUDYING PROTEIN D5-GBD FIXATION TO SERUM ANTIBODIES, METHOD FOR MAKING SPECIFIC PROTEIN D5-GBD ANTIBODIES

摘要

FIELD: medicine. ^ SUBSTANCE: invention represents plasmid DNA pD5spGBD enabling the expression of a recombinant antigen - protein LigA L. interrogans domain 5 in chimeric protein D5-GBD and 1,3--glucan-binding domain (GBD), and a method for making a based subunit engineered leptospirosis vaccine. ^ EFFECT: invention allows ensuring the development of an intensive immune response and high level of antibodies synthesis with no additional adjuvants. ^ 6 cl, 1 dwg, 6 ex