| 摘要 |
A microscopy imaging system is disclosed that includes a light source system, focusing optics, an optical detector and a processor. The light source system is for providing an excitation beam at a center optical frequency ωe and for providing a stimulation beam at a center optical frequency ωs. The focusing optics is for directing and focusing the excitation beam toward a common focal volume such that a sample may be excited to an electronic excited state, and for directing and focusing the stimulation beam toward the common focal volume such that stimulated emission induced from the electronic excited state results in an increase in intensity of the stimulated beam. The optical detector is for detecting an increase in a radiation field at the center optical frequency ωs from stimulated emission from the common focal volume and for providing a detector signal. The processor is for receiving the detector signal and for providing a pixel of an image for the microscopy imaging system. In certain embodiments, the stimulated emission imaging allows detection and imaging of non-fluorescent chromophores such as drug molecules, small dye molecules and proteins in living cells, tissues and organisms with intrinsic 3D optical sectioning and high sensitivity.
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