发明名称 |
Recombinant alphavirus-based vectors with reduced inhibition of cellular macromolecular synthesis |
摘要 |
<p>Isolated nucleic acid molecules are disclosed, comprising an alphavirus nonstructural protein gene which, when operably incorporated into a recombinant alphavirus particle, eukaryotic layered vector initiation system, or RNA vector replicon, has a reduced level of vector-specific RNA synthesis, as compared to wild-type, and the same or greater level of proteins encoded by RNA transcribed from the viral junction region promoter, as compared to a wild-type recombinant alphavirus particle. Also disclosed are RNA vector replicons, alphavirus vector constructs, and eukaryotic layered vector initiation systems which contain the above-identified nucleic acid molecules.
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申请公布号 |
EP1997900(A3) |
申请公布日期 |
2010.10.06 |
申请号 |
EP20080075018 |
申请日期 |
1997.04.04 |
申请人 |
NOVARTIS VACCINES AND DIAGNOSTICS, INC.;WASHINGTON UNIVERSITY |
发明人 |
DUBENSKY, THOMAS W., JR.;POLO, JOHN M.;BELLI, BARBARA A.;SCHLESINGER, SONDRA;DRYGA, SERGEY A.;FROLOV, ILYA |
分类号 |
C12N15/09;C12N15/86;A61K39/00;A61K48/00;C07K14/18;C12N5/10;C12N7/00;C12N7/04;C12N15/33;C12P21/00 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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