RIFT VALLEY FEVER COMPETITION ELISA USING MONOCLONAL ANTIBODIES AGAINST RECOMBINANT N PROTEIN

摘要

PURPOSE: A competitive ELISA using a monoclonal antibody to a recombinant N protein is provided to shorten diagnosis time. CONSTITUTION: A competitive ELISA using recombinant N protein and monoclonal antibody 1-59 is used for diagnosing rift valley fever. A method for diagnosing rift valley fever comprises: a step of attaching a recombinant N protein antigen of rift valley fever virus on an ELISA plate; a step of removing the recombinant N protein antigen which is not attached on the plate; a step of competing 1-59 monoclonal antibodies to the recombinant N protein; and a step of reacting the secondary antibody to the monoclonal antibody conjugated with a target material.