| 摘要 |
The present invention relates to a fluidic apparatus for a reaction for differentiating viable cells. The fluidic apparatus comprises a cartridge comprising a substantially optically transparent reaction chamber configured to expose to a light source a sample comprising cells and a phenanthridium compound capable of preferentially penetrating dead or membrane-compromised cells over viable and/or substantially intact cells to intercalate with at least one nucleic acid molecule and covalently binding to the nucleic acid molecule on exposure to a light source, at least one inlet in fluid communication with the reaction chamber; and at least one outlet in fluid communication with the reaction chamber; and at least one light source configured to transmit light to the reaction chamber. The fluidic apparatus may be automated. The invention also relates to a method for differentiating viable cells and membrane-compromised cells with the fluidic apparatus. |
