发明名称 |
Methods for producing soluble multi-membrane-spanning proteins |
摘要 |
Methods for producing membrane-spanning polypeptides in high yields, with native conformation, and/or in soluble form include solubilizing in non-ionic or zwitterionic detergents as well as use of promoters and expression vectors for expressing high yields of membrane-spanning polypeptides in bacterial cells. Mutated promoters provide tight control of membrane-spanning polypeptides in bacterial cell hosts. |
申请公布号 |
ZA200704934(B) |
申请公布日期 |
2010.09.29 |
申请号 |
ZA20070004934 |
申请日期 |
2005.12.22 |
申请人 |
GENENTECH, INC. |
发明人 |
ERNST, JAMES A;YANSURA, DANIEL G;KIM, HOK SEON |
分类号 |
C07K;C12N;G01N |
主分类号 |
C07K |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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