A CHIP FOR MEASURING A MATRIX METALLOPROTEINASE ACTIVITY AND A METHOD USING THE SAME

摘要

PURPOSE: A protein chip for measuring MMP activation, in which a protein is fixed on a solid substrate and a method for measuring MMP activation using the same are provided. CONSTITUTION: A method for measuring MMP(matrix metalloproteinase) activation comprises: a step of preparing the protein chip; a step of contacting a sample containing MMP with the array chip; and a step of confirming substrate amount of decomposed MMP by SPR signal measurement to measure MMP activation. The MMP is selected from MMP-1 to MMP-23. The sample additionally contains Brij-35, Ca2^+ and Zn^2+. The MMP substrate is gelatin, proteoglycan, fibronectin, laminin, or elastin. The SPR signal measurement is performed using SPR biosensor or SPR(surface plasmon resonance imaging).