摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method of accurately and easily measuring C3a-desArg in biological samples, and to provide polypeptide for detection and a measurement kit ideal for the method. Ž<P>SOLUTION: The method of measuring C3a-desArg concentration includes: a process for forming a coupled body of polypeptide for detection and a specific antibody by adding the polypeptide for detecting C3a-desArg, a biological sample, and an anti C3a-desArg specific antibody to a reaction solution and for measuring quantity of polypeptide for detection in the coupled body; a process for forming a coupled body of polypeptide for detection and a specific antibody, by adding the polypeptide for detection, a polypeptide solution for competition having a known concentration, and a specific antibody to the reaction solution, for measuring quantity of polypeptide for detection in the coupled body, and for correlating the quantity of polypeptide for competition added to the reaction solution to the quantity of polypeptide for detection in the coupled body; and a process for calculating C3a-desArg concentration in the biological sample from the quantity of polypeptide for detection measured in the process, based on the correlation obtained. Ž<P>COPYRIGHT: (C)2010,JPO&INPIT Ž
|