MICROFLUIDIC DEVICE FOR TRAPPING SINGLE CELL

摘要

It is to provide a microfluidic device which separates and captures with high efficiency a large amount of cells in a sample at one-cell level without damaging the cells by utilizing a microfabrication technology. The device sequentially comprises: an upper substrate consisting of a plastic plate provided with a groove for forming a microchannel communicating a sample-supply opening and a sample-discharge opening; a plastic upper flat plate forming the microchannel by cooperating with the upper substrate, the plate being provided with an aperture for a micromesh; a holding plate holding a plastic micromesh having a plurality of micro through-pores for capturing cells; a plastic lower flat plate provided with an aperture for suctioning; and a lower substrate consisting of a plastic flat plate provided with a groove for forming a suction channel, wherein the micro through-pores for capturing cells of the micromesh have an inverted mortar-like shape or a cylindrical shape, and wherein the upper flat plate is constituted by a softer plastic as compared to the upper substrate.