Verfahren zum Erhalten von natürlichem Astaxanthin aus Eiern und Gonaden aus Schnecken

摘要

The present invention relates to a process for obtaining astaxanthin from eggs and/or gonads of snails comprising the steps of:
(a) grinding the eggs and/or gonads;
(b) mixing the ground eggs and/or gonads sample with a first solvent while conducting sonication;
(c) removing the solid components in the mixture to obtain a glycol-lipo-carotenoprotein solution;
(d) contacting the glycol-lipo-carotenoprotein solution with a proteinase in the dark under N 2 atmosphere so that the proteins are digested;
(e) removing the digested proteins to obtain a glycol-lipo-carotenoid solution;
(f) removing lipids and saccharide compounds from the glycol-lipo-carotenoid solution to obtain a carotenoid solution;
(g) extracting the carotenoid solution with a second solvent to obtain an astaxanthin-containing solution; and
(h) purifying astaxanthin from the astaxanthin-containing solution.