发明名称 |
Hybrid proteins that migrate retrogradely and transynaptically into the CNS |
摘要 |
The non-toxic proteolytic C fragment of tetanus toxin (TTC peptide) has the same ability to bind nerve cells and be retrogradely transported through a synapse as the native toxin. A hybrid protein encoded by the IacZ-TTC gene fusion retains the biological functions of both proteins in vivo, i.e. retrograde transynaptic transport of the TTC fragment andβ-gal enzymatic activity. After intramuscular injection, enzymatic activity could be detected in motoneurons and connected neurons of the brainstem areas. This strategy is useful for the delivery of a biological activity to neurons from the periphery to the central nervous system. Such a hybrid protein can also be used to map synaptic connections between neural cells.
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申请公布号 |
US2010239534(A1) |
申请公布日期 |
2010.09.23 |
申请号 |
US20080213982 |
申请日期 |
2008.06.26 |
申请人 |
INSTITUT PASTEUR |
发明人 |
COEN LAURENT;PINZOLAS ROSARIO OSTA;BRULET PHILIPPE |
分类号 |
A61K35/00;A61K31/713;A61K38/00;A61K47/48;A61P21/02;C07K14/33 |
主分类号 |
A61K35/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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