发明名称 |
Recombinant murine leukemia virus reverse transcriptases, the genes encoding and the method for expressing it |
摘要 |
This invention provides recombinant murine leukemia virus reverse transcriptases, genes encoding these proteins and expression methods. The said murine leukemia virus reverse transcriptases are a series of MLV-RT proteins wherein the 84th amino acid residue (Q84) from the N-terminus is replaced with amino acid X, which is an amino acid with a side chain shorter than glutamine. The said murine leukemia virus reverse transcriptases have higher enzyme activity and processivity than the wild type enzyme, and are expected to be widely used in the field of biotechnology for cDNA synthesis.
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申请公布号 |
US7794996(B2) |
申请公布日期 |
2010.09.14 |
申请号 |
US20040585863 |
申请日期 |
2004.01.13 |
申请人 |
THE INSTITUTE OF BIOPHYSICS, CHINESE ACADEMY OF SCIENCES |
发明人 |
GAO GUANGXIA;LIU SHUFENG |
分类号 |
C12N9/12;C07H21/04;C12N1/20;C12N15/00;C12N15/54;C12P21/04;C12Q1/00;C12Q1/68 |
主分类号 |
C12N9/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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