发明名称 |
Vectors for expressing in vivo biotinylated recombinant proteins |
摘要 |
The present invention provides compositions and methods for generating in vivo biotinylated, secreted recombinant polypeptides comprising recombinantly co-expressing in a yeast cell (a) a secreted recombinant polypeptide comprising a biotin accepting site; and (b) a biotin ligase fused to the golgi localization domain so that the biotin ligase is localized to the golgi of the yeast cell where it acts on the biotin-accepting site of the secreted polypeptide as it transits the secretory system.
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申请公布号 |
US7795411(B2) |
申请公布日期 |
2010.09.14 |
申请号 |
US20070669811 |
申请日期 |
2007.01.31 |
申请人 |
FRED HUTCHINSON CANCER RESEARCH CENTER |
发明人 |
SCHOLLER NATHALIE;GARVIK BARBARA |
分类号 |
C07H21/04;C12P9/00;C12P21/06 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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