| 摘要 |
FIELD: medicine. ^ SUBSTANCE: viral particle consists of non-alphavirus structural elements and contains an alphavirus vector replication-defective by deletion or substitution by at least one transgene. Besides said particle contains structural elements not coded by an alphavirus vector genome. A method for preparing said particle according to the invention implies the expression in trans of the genes coding the non-alphavirus structural elements, and the alphavirus vector, in a cell line and then recovery of the viral particles found in the cell culture supernatant. ^ EFFECT: invention allows preparing the viral particles with lower recombination risk. ^ 24 cl, 5 dwg, 3 tbl, 2 ex |
