QUANTITATION OF SERUM CELL SIGNALING PATHWAY PROTEINS

摘要

Methods are provided to concentrate and detect low abundance serum proteins, such as kinase and cell signaling pathway proteins using hydrogel nanoparticles to sequester and concentrate a protein of interest, and reverse phase protein microarrays to quantitate the protein The markers identified by the described methods have wide applicability in diagnosis and treatment of a variety of diseases including myocardial infarction, pulmonary embolus, stroke, and organ infarction