| 摘要 |
FIELD: medicine. ^ SUBSTANCE: diploid cell cultures are reduced and collected by propagation in a monolayer in an Eagle's growth medium. The conditioned growth medium is separated from the formed cell layer by sterile bottling. The cell layer is removed, washed by centrifugation. The prepared cell suspension is reduced to the required and bottled in the sterile environment. The conditioned growth medium bottles and cell suspension bottles are consistently single frozen to temperature -20C and higher and thawed at temperature +25C and lower, and bottled in the sterile environment. The conditioned growth medium and cell suspension are frozen in open bottles at temperature minus 50C and lower, kept in the frozen environment for at least 48 hours and then lyophilised in two stages. The first stage involves desorption at temperature minus 50C to 0C for at least 20 hours; and the second stage requires sublimation for at least 20 hours at temperature 0C to 30C; the finished lyophilised bottles are closed in the sterile environment. The invention allows preparing the lyophilised preparations effective in treatment of deep vast burning wounds, frostbites, degrees III (a, b) and IV subdermal burns, treatment of oral and nasopharyngeal mucosa, are storage-stable at temperature +4C for at least 12 months. ^ EFFECT: higher efficacy of the preparations for treatment of diseases. ^ 1 dwg, 3 tbl, 2 cl |
