DETECTION SYSTEM AND METHOD FOR HIGH SENSITIVITY FLUORESCENT ASSAYS
摘要
This invention relates to a detection system for measuring a fluorescent signal in a fluorescent assay. The system comprises a probe having a small sensing surface bound with a fluorescent label, and a light source and a detector both mounted at the proximal side of the sensing surface of the substrate. The invention also relates to a method for detecting an analyte in a liquid sample using a probe tip having a small surface area ( =5 mm) and a high molecular weight polymer ( =1 MD) having multiple binding molecules and multiple fluorescent labels. The binding reaction is accelerated by flowing the reaction solutions laterally and moving the probe tip up and down in the reaction vessels. The invention furthers relates to a fluorescent labeling composition comprising a cross-linked Ficoll molecule having a plurality of binding molecules and a plurality of fluorescent labels.
申请公布号
WO2010101931(A2)
申请公布日期
2010.09.10
申请号
WO2010US25938
申请日期
2010.03.02
申请人
ACCESS MEDICAL SYSTEM CO., LTD.;TAN, HONG;ZUK, ROBERT F.;TAN, YUSHAN;CAO, ERHUA;XIA, MIN;CHEN, JUN
发明人
TAN, HONG;ZUK, ROBERT F.;TAN, YUSHAN;CAO, ERHUA;XIA, MIN;CHEN, JUN