| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for selecting repertoires of polypeptides by using generic ligands and target ligands. SOLUTION: The present invention relates to the method for selecting, from a repertoire of polypeptides, a population of functional polypeptides which bind a target ligand in a first binding site and to a generic ligand in a second binding site (wherein the generic ligand is capable of binding a functional member of the repertoire regardless of the target ligand specificity), and the method includes the steps of: (a) bringing the repertoire into contact with the generic ligand and selecting functional polypeptides bound thereto; and (b) bringing the selected functional polypeptides into contact with the target ligand and selecting a population of polypeptides with bind to the target ligand. COPYRIGHT: (C)2010,JPO&INPIT
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