| 摘要 |
An apparatus and method is provided for the rapid detection, identification and/or enumeration of one or more live target microorganisms growing on solid nutrient media or filtration membrane. Microcolonies or colonies attach to a porous transfer permeable membrane loaded with immobilized antibodies specific for target microorganisms. Non-specific cells are washed out of the permeable membrane and the membrane then is placed on a container containing a chromogenic and/or a fluorogenic substrate and incubated to allow coloration and subsequent identification and enumeration of color spots of targeted microorganisms and patterns of microcolonies or colonies.
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