发明名称 |
FILAMENTOUS FUNGAL MUTANTS WITH IMPROVED HOMOLOGOUS RECOMBINATION EFFICIENCY |
摘要 |
The present invention relates to a method for increasing the efficiency of targeted integration of a polynucleotide to a pre-determined site into the genome of a filamentous fungal cell with a preference for NHR, wherein said polynucleotide has a region of homology with said pre-determined site, comprising steering an integration pathway towards HR. The present invention also relates to a mutant filamentous fungus originating from a parent cell, said mutant having an HR pathway with elevated efficiency and/or an NHR pathway with a lowered efficiency and/or a NHR/HR ratio with decreased efficiency as compared to said HR and/or NHR efficiency and/or NHR/HR ratio of said parent cell under the same conditions. |
申请公布号 |
EP1733040(B1) |
申请公布日期 |
2010.09.08 |
申请号 |
EP20050740129 |
申请日期 |
2005.03.31 |
申请人 |
DSM IP ASSETS B.V. |
发明人 |
DEKKER, PETRUS, JACOBUS, THEODORUS;BERG, VAN DEN, MARCO ALEXANDER |
分类号 |
C12N15/90;C07K14/37;C07K14/38;C07K14/385;C12N5/00;C12N15/80;C12P21/02;C12P23/00;C12P37/00 |
主分类号 |
C12N15/90 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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