ANTIBODY AGAINST SELENOPROTEIN O AVAILABLE FOR WESTERN BLOTTING AND IMMUNOPRECIPITATION AND METHOD FOR PREPARATION THEREOF
摘要
PURPOSE: An antibody to a specific region of selenoprotein O is provided to use in western blotting and immunoprecipitation and to quantify and qualify selenoprotein O. CONSTITUTION: An isolated peptide comprises an amino acid sequence of sequence number 1. The peptide contains a cystein at N-terminal for KLH conjugation. The peptide is modified by amidation of C-terminal. An antibody which specifically binds to the peptide with an amino acid of sequence number 1 specifically binds to selenoprotein O. The antibody is used in western blotting or ELISA analysis. A method for detecting a specific polypeptide in a sample comprises: a step of contacting the antibody to the sample; a step of forming complex of the specific polypeptide and antibody; and a step of detecting the complex.
申请公布号
KR20100098039(A)
申请公布日期
2010.09.06
申请号
KR20090017002
申请日期
2009.02.27
申请人
INDUSTRY FOUNDATION OF CHONNAM NATIONAL UNIVERSITY
发明人
LEE, SEUNG ROCK;KIM, ICK YOUNG;LEE, TAE HOON;CHAY, KEE OH;AHN, YOUNG HEE;HAN, SEONG JEONG;KIM, IN YOUNG