?-GLUCAN-DEFICIENT GENE IN BARLEY, SYNTHETIC GENE, AND USE OF SAME

摘要

Disclosed are: a ß-glucan-deficient gene in a barley; genomic DNA and cDNA of a ß-glucan synthesis gene; a barley having genomic DNA for a ß-glucan-deficient gene; a method for breeding the barley; an alcohol or fermented food production method, which utilizes grains of a ß-glucan-reduced or -deficient barley; and an animal feed composition. Specifically disclosed are: genomic DNA for a ß-glucan-deficient gene, which is DNA comprising a nucleotide sequence having a 90% or higher homology with the nucleotide sequence depicted in SEQ ID NO:1 and capable of being transcribed and translated into a protein lacking a ß-glucan synthesis activity; and a breeding method comprising steps of determining a barley as having a ß-glucan-deficient gene when a nucleotide corresponding to the 4275th nucleotide or the 2385th nucleotide in a gene comprising the nucleotide sequence depicted in SEQ ID NO:4 that is located adjacent to a centromere of chromosome-7H in the barley is mutated from G to A, and selecting the barley.