发明名称 |
NUCLEOTIDE TRIPHOSPHATE WITH AN ELECTROACTIVE LABEL CONJUGATED TO THE GAMMA PHOSPHATE |
摘要 |
A nucleotide triphosphate (NTP) participates in a phosphorylation reaction, wherein a phosphate group is transferred from the NTP to a substrate by a kinase. Provision in a kinase reaction of a NTP whose gamma phosphate is conjugated to an electroactive label results in the transfer of the gamma phosphate-electroactive label conjugate from the NTP to the substrate. The electroactive label is an organic moiety such as a quinone or a nitrohetercycle, or is a metallocene such as a ferrocene or a cobaltocene. Upon transfer of the gamma phosphate-electroactive label conjugate to an electrode-bound substrate by a kinase, the phosphorylation event is detected electrochemically by cyclic voltammetry. Phosphorylation can also be detected by mass spectrometry of a substrate carrying the electroactive label-conjugated gamma phosphate. NTP comprising the gamma phosphate-electroactive label conjugate is used in methods of detecting the presence of a kinase in a sample, screening candidate compounds that modulate kinase activity, and in methods of diagnosing a disease associated with a kinase.
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申请公布号 |
WO2009039639(A8) |
申请公布日期 |
2010.09.02 |
申请号 |
WO2008CA01690 |
申请日期 |
2008.09.26 |
申请人 |
KRAATZ, BERNIE;KERMAN, KAGAN;SONG, HAIFENG |
发明人 |
KRAATZ, BERNIE;KERMAN, KAGAN;SONG, HAIFENG |
分类号 |
C12Q1/48;C07H19/10;C07H19/20;C07K16/44;C12Q1/00;C40B30/08;G01N27/403 |
主分类号 |
C12Q1/48 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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