发明名称 |
Probes for detecting protein nuclear transport and method for detecting and quantifying protein nuclear transport using the same |
摘要 |
A convenient and highly accurate method for detecting protein nuclear transport induced by an endogenous or exogenous substance in local areas of living cells or animals is provided. The method uses a pair of probes for detecting protein nuclear transport, comprising Probe I and Probe II. In Probe I, a protein whose nuclear transport is to be detected or quantified is connected to an N-terminal end or a C-terminal end of a fusion protein [intein-C/reporter protein-C] wherein at least a C-terminal side polypeptide of an intein and a C-terminal side polypeptide of a reporter protein are connected in this order, and in Probe II, a nuclear localization signal is connected to an N-terminal end or a C-terminal end of a fusion protein [reporter protein-N/intein-N] wherein at least the remaining N-terminal side polypeptide of the reporter protein and the remaining N-terminal side polypeptide of the intein are connected in this order.
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申请公布号 |
US2010221719(A1) |
申请公布日期 |
2010.09.02 |
申请号 |
US20100656138 |
申请日期 |
2010.01.19 |
申请人 |
UMEZAWA YOSHIO;OZAWA TAKEAKI |
发明人 |
UMEZAWA YOSHIO;OZAWA TAKEAKI |
分类号 |
C12Q1/68;C12N15/09;C12N15/62;G01N33/15;G01N33/50 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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