CELL LINE EXPRESSING 5-HT6R AND HIGH-THROUGHPUT SCREENING METHOD OF SELECTIVE LIGANDS BY USING IT

摘要

PURPOSE: A transformed cell line which expresses human serotonin 6 receptor(5-HT6R) is provided to stably express Myc-5-HT6R on the cell surface and to use in screening human serotonin 6-receptor-specific ligand by fluorescence imaging. CONSTITUTION: A HELA cell line stably expresses Myc-5-HT6R by transducing vector containing Myc-labeled 5-HT6R. The Myc-labeld 5-HT6R gene is a gene of sequence number 1. A HELA cell line is Hela cell(deposit number KCLRF=BR-00200) which stably expresses Myc-5-HT6R. A method for screening 5-HT6R ligand of high efficiency comprises: a step of treating test chemical to a cell line; a step of measuring 5-HT6R activity of the cell line; and a step of selecting test chemical which increases or decreases 5-HT6R in the cell line.