| 摘要 |
The present invention provides a system relating to periodically and synchronously switching between photon beams from at least two emitters (10,12) that are each projecting photons into an optical trap region (18). In one embodiment, the system comprises a first emitter (12) capable of emitting photons to form an optical trapping region (18). The system further comprises a second emitter (10) capable of emitting photons into the trapping region. The system also comprises a modulator (14,16) capable of periodically coupling photons from at least one of the first or second emitter to the trapping region. In one aspect, the invention provides at least two modulators (14,16) for periodically and synchronously operating to couple photons from the first and second emitter to the trapping region. The invention also provides a method of using such a system, for force -fluorescence microscopy. Such a system/method can reduce photo bleaching by switching between the highflux trapping beam (12) and the lower- intensity fluorescence laser (12). |
