PROCESS FOR DIFFERENTIATION OF VASCULAR ENDOTHELIAL PROGENITOR CELLS FROM EMBRYOID BODIES DERIVED FROM EMBRYONIC STEM CELLS USING HYPOXIC MEDIA CONDITION

摘要

The present invention provides a process for differentiation of vascular endothelial progenitor cells from embryoid bodies derived from embryonic stem cells, the process comprising: (a) treating a culture medium comprising embryoid bodies derived from embryonic stem cells such that the concentration of oxygen dissolved in the culture medium is in the range of about 1 ppm to about 5 ppm; (b) culturing the culture medium prepared in step (a) in an incubator in which the oxygen (O2) tension is equal to or less than about 15% to differentiate the embryoid bodies into vascular endothelial progenitor cells; and (c) isolating the vascular endothelial progenitor cells from the culture medium obtained in step (b).