EVALUATION OF METABOLIC DIFFERENCES BETWEEN URINE SAMPLES BY QUANTITATIVE STEROID SIGNATURE

摘要

PURPOSE: A method for analyzing difference of steroid hormone bio metabolite from two sample groups is provided to general metabolism tendency. CONSTITUTION: A method for analyzing difference of steroid hormone bio metabolite from two sample groups comprises: a step of extracting steroid hormone bio metabolite including male hormone, female hormone, corpus luteum hormone, sterol, and adrenal cortical hormone; a step of extracting the extracts again using an organic solvent; a step of measuring concentration of steroid hormone bio metabolite in urine by gas chromatography-mass spectrometry; and a step of comparing the concentration of liver steroid hormone bio metabolite between two samples. The organic solvent is a mixture solvent containing ethyl acetate and normal hexane.