| 摘要 |
PROBLEM TO BE SOLVED: To provide an assay method capable of substantially shortening assay time when a physiologically active substance of biological origin such as an endotoxin or β-D-glucan is to be detected or its concentration is to be measured through the use of the reaction between the physiologically active substance and LAL and to provide an assay apparatus using the same. SOLUTION: The aggregation start time of a liquid mixture of an assay sample, an object of the detection of a physiologically active substance or the measurement of the concentration of the same, with LAL is detected. On the basis of this aggregation start time, the physiologically active substance is detected or the concentration thereof is measured. The liquid mixture of the assay sample with LAL is stirred with, for example, a magnetic stirrer to form gel particles, and the intensity of scattered light of laser light scattered by the gel particles is measured. A frequency distribution of fluctuations in the intensity of the scattered light is obtained. On the basis of the temporal change in the frequency distribution shape, the aggregation start time of the liquid mixture of the assay sample with LAL is detected. COPYRIGHT: (C)2010,JPO&INPIT |
