发明名称 |
TRANSIENTLY BONDING DRAG-TAGS FOR SEPARATION MODALITIES |
摘要 |
The invention relates transiently attaching drag-tags to molecules during electrophoresis. The invention includes running buffers having drag-tags that transiently attach to lipophilic moieties attached to the molecules. The lipophilic moieties can be covalently or ionically bonded to the molecules. One particular aspect of the invention is a nucleoside analog or a nucleic acid analog comprising a lipophilic moiety. The invention is also directed to methods of separating molecules that comprise a lipophilic moiety. The methods generally comprise transiently attaching a drag-tag to the lipophilic moiety during a separation modality. These methods can be used to separate the molecules by size or weight, to measure a hydrodynamic radius of a drag-tag, or to separate a plurality of drag-tag by their hydrodynamic radius.
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申请公布号 |
US2010213059(A1) |
申请公布日期 |
2010.08.26 |
申请号 |
US20080665994 |
申请日期 |
2008.06.23 |
申请人 |
CARNEGIE MELLON UNIVERSITY |
发明人 |
SCHNEIDER JAMES W.;GROSSER SHANE T.;SAVARD JEFFREY M. |
分类号 |
G01N27/26;C07H21/00;C07K1/00 |
主分类号 |
G01N27/26 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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