METHOD FOR THE SCREENING PROTEASOME OF ACTIVITY-MODULATING AGENTS AND MEANS FOR CARRYIING OUT SAID METHOD

摘要

An in cellulo method for the screening of proteasome activity-modulating agents, includes the following steps of: a) bringing into contact the candidate agent with the yeast cell expressing in their nucleus a fusion protein comprising: (i) a p21 polypeptide selected from the p21WAF1/Cip1 polypeptide and the p21[6KR]WAF1/Cip1 polypeptide; and (ii) at least one detectable protein; b) quantifying the first detectable protein in the yeast cells, at the end of at least one predetermined time interval after bringing into contact the candidate agent with the cells; c) comparing the value obtained at the step (b) with a control value obtained when the step (a) is performed in the absence of the candidate agent.