发明名称 |
TRANSLATION REGULATION SYSTEM IN CELL OR ARTIFICIAL CELL MODEL BY USING LOW-MOLECULAR-WEIGHT RNA |
摘要 |
<p>An object of the present invention is to construct an mRNA which specifically responds to a short RNA sequence and can activate, repress, and regulate the translation of the desired gene, and to construct an artificial cell model system using a liposome comprising the mRNA and a cell-free translational system encapsulated therein. The present invention provides: an mRNA comprising a target RNA-binding site located immediately 5' to the ribosome-binding site, and a nucleotide sequence located 5' to the target RNA-binding site, the nucleotide sequence being complementary to the ribosome-binding site; an mRNA comprising a small RNA-binding site located 3' to the start codon, and a nucleotide sequence located 3' to the small RNA-binding site, the nucleotide sequence encoding a protein; and a liposome comprising any of these mRNAs encapsulated therein.</p> |
申请公布号 |
EP2221371(A1) |
申请公布日期 |
2010.08.25 |
申请号 |
EP20080852782 |
申请日期 |
2008.11.21 |
申请人 |
JAPAN SCIENCE AND TECHNOLOGY AGENCY |
发明人 |
INOUE, TAN;SAITO, HIROHIDE;KATO, YUSHO;YOSHIKAWA, KENICHI;YAMADA, AYAKO;YAMANAKA, TORU |
分类号 |
C12N15/09;C12N15/11;C12N15/63;C12P21/02 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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