PROCESS FOR PRODUCTION OF NON-NATURAL PROTEIN HAVING ESTER BOND THEREIN

摘要

A non-natural protein having at least one ester bond in its polypeptide main chain is synthesized by using an in vivo translation system in a ribosome. The following components (a) to (c) are expressed in a cell or an cell extraction solution in the presence of an ±-hydroxy acid: (a) an aminoacyl-tRNA synthetase which can activate the ±-hydroxy acid; (b) suppressor tRNA which can bind to the ±-hydroxy acid in the presence of the aminoacyl-tRNA synthetase; and (c) a gene encoding a desired protein having a nonsense mutation or a frame-shift mutation at a desired site.