发明名称 |
ORGANISM IDENTIFICATION PANEL |
摘要 |
Methods and containers are provided for identifying a species, illustratively a bacterial species. Illustrative methods comprise amplifying various genes in the nucleic acid from the bacterial species in a single reaction mixture using pairs of outer first-stage primers designed to hybridize to generally conserved regions of the respective genes to generate a plurality of first-stage amplicons, dividing the reaction mixture into a plurality of second-stage reactions, each using a unique pair of second-stage primers, each pair of second-stage primers specific for a target bacterial species or subset of bacterial species, detecting which of the second-stage reactions amplified, and identifying the bacterial species based on second-stage amplification. Methods for determining antibiotic resistance are also provided, such methods also using first-stage primers for amplifying genes known to affect antibiotic resistance a plurality of the second-stage reactions wherein each pair of second-stage primers specific for a specific gene for conferring antibiotic resistance. |
申请公布号 |
EP2145018(A4) |
申请公布日期 |
2010.08.25 |
申请号 |
EP20080744843 |
申请日期 |
2008.04.01 |
申请人 |
UNIVERSITY OF UTAH RESEARCH FOUNDATION;IDAHO TECHNOLOGY |
发明人 |
BLASCHKE-BONKOWSKY, ANNE, JEANNETTE;PORITZ, MARK, AARON |
分类号 |
C12P19/34;B01L3/00;B01L7/00;C12Q1/68;G01N33/00 |
主分类号 |
C12P19/34 |
代理机构 |
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