摘要 |
<p>This invention relates to new insights into the pathogenesis of pancreatic cancers by analyzing the genes altered in 24 pancreatic tumors. First, we determined the sequences of 23,781 transcripts, representing 20,583 protein-encoding genes, in DNA from these tumors. Second, we searched for homozygous deletions and amplifications using microarrays querying one million single nucleotide polymorphisms in each sample. Third, we analyzed the transcriptomes of the same samples using SAGE and next-generation sequencing-by-synthesis technologies We found that pancreatic cancers contain an average of 63 genetic alterations, of which 49 are point mutations, 8 are homozygous deletions, and 6 are amplifications. Further analyses revealed a core set of 12 regulatory processes or pathways that were each genetically altered in 70 % to 100 % of the samples. The data suggest that dysregulation of this core set of pathways is responsible for the major features of pancreatic tumorigenesis.</p> |
申请人 |
THE JOHNS HOPKINS UNIVERSITY;VOGELSTEIN, BERT;KINZLER, KENNETH, W.;PARSONS, D., WILLIAMS;JONES, SIAN;ZHANG, XIAOSONG;LIN, JIMMY CHANG-HO;LEARY, REBECCA J.;ANGENENDT, PHILIPP;PAPADOPOULOS, NICKOLAS;VELCULESCU, VICTOR;PARMIGIANI, GIOVANNI;KARCHIN, RACHEL;KERN, SCOTT;HRUBAN, RALPH;ESHLEMAN, JAMES R.;GOGGINS, MICHAEL;KLEIN, ALISON |
发明人 |
VOGELSTEIN, BERT;KINZLER, KENNETH, W.;PARSONS, D., WILLIAMS;JONES, SIAN;ZHANG, XIAOSONG;LIN, JIMMY CHANG-HO;LEARY, REBECCA J.;ANGENENDT, PHILIPP;PAPADOPOULOS, NICKOLAS;VELCULESCU, VICTOR;PARMIGIANI, GIOVANNI;KARCHIN, RACHEL;KERN, SCOTT;HRUBAN, RALPH;ESHLEMAN, JAMES R.;GOGGINS, MICHAEL;KLEIN, ALISON |