REFOLDING AND RECONSTITUTION OF HUMAN RECOMBINANT BAX INHIBITOR-1 INTO LIPOSOMES FROM INCLUSION BODIES EXPRESSED IN ESCHERICHIA COLI

摘要

PURPOSE: A method for refolding and recombining recombinant Bas inhibitor-1(BI-1) is provided to use for in vitro functional analysis of BI-1 and to induce Ca^2+ release. CONSTITUTION: A method for producing a liposome containing BI-1 protein comprises: a step of separating an inclusion body containing BI-1 protein; a step of dissolving charotropic agent of first concentration, reductant, and amphoteric surfactant in a first buffer; a step of mixing the first buffer with phospholipid; and a step of exchanging the first buffer to a second buffer. The charotropic agent is urea or guanidiniumchloride.