USE OF PEI FOR THE IMPROVEMENT OF ENDOSOMAL RELEASE AND EXPRESSION OF TRANSFECTED NUCLEIC ACIDS, COMPLEXED WITH CATIONIC OR POLYCATIONIC COMPOUNDS

摘要

The present invention is directed to complexed precomplexed nucleic acids, preferably mRNAs, which have been precomplexed in a first step with PEI in an N/P ratio between 0.05 and 2, preferably in an N/P ratio between 0.1 and 1, and which have been further complexed in a second step with a cationic compound. The present invention is furthermore directed to the use of such complexed precomplexed nucleic acids for the improvement of the endosomal release of nucleic acids and optionally the improvement of expression of an encoded protein or peptide. It is also directed to compositions comprising such complexed precomplexed nucleic acids and to the use of such complexed precomplexed nucleic acids or compositions thereof for gene therapy and/or the treatment of various diseases as mentioned herein, e.g. by vaccination. The present invention is also directed to methods for preparing and administering these complexed precomplexed nucleic acids or compositions thereof and to kits, comprising these complexed precomplexed nucleic acids or compositions thereof.