发明名称 |
METHOD FOR DETECTION OF HOP LATENT VIRUS, PRIMER SET FOR THE DETECTION, AND KIT FOR THE DETECTION |
摘要 |
The invention provides a detection method for hop latent virus that comprises an extraction step in which hop latent virus RNA is extracted from a specimen, an amplification step in which a primer set containing 4 different oligonucleotides consisting of the nucleotide sequences represented by SEQ ID NO: 1-4 of the Sequence Listing is used for amplification of cDNA by RT-LAMP using the RNA as template, and a judging step in which hop latent virus is judged to be present when amplification of cDNA containing the nucleotide sequence represented by SEQ ID NO: 8 of the Sequence Listing has occurred in the amplification step.
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申请公布号 |
US2010203500(A1) |
申请公布日期 |
2010.08.12 |
申请号 |
US20070517073 |
申请日期 |
2007.11.26 |
申请人 |
SAPPORO BREWERIES LIMITED |
发明人 |
OKADA YUKIO;ITOGA YUTAKA |
分类号 |
C12Q1/70 |
主分类号 |
C12Q1/70 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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