发明名称 |
Purification of Factor VIII Using a Mixed-Mode or Multimodal Resin |
摘要 |
A method for purifying a recombinant protein using a multimodal or mixed mode resin containing ligands which comprise a hydrophobic part and a negatively charged part is described. The invention is advantageous in that it is a single step chromatographic process which does not require adjustment of pH or conductivity during loading step and results in high yield and potency. The process is used for the purification of recombinant compositions of coagulation factor, particularly recombinant Factor VIII.
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申请公布号 |
US2010204452(A1) |
申请公布日期 |
2010.08.12 |
申请号 |
US20080668530 |
申请日期 |
2008.07.11 |
申请人 |
NOVO NORDISK HEALTH CARE AG |
发明人 |
BANG SUSANNE;THIM LARS;KARLSSON JOHAN |
分类号 |
C07K1/14 |
主分类号 |
C07K1/14 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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