TRANSFORMATION SYSTEM IN TOLYPOCLADIUM NIVEUM

摘要

PURPOSE: A novel transformation system in Tolypocladiium niveum is provided to enhance cyclosporine A productivity and to industrially and medically use. CONSTITUTION: A method for preparing transformed Tolypocladium niveum comprises: a step of forming protoplast of Tolypocladium niveum; a step of preparing a recombinant vector in which foreign gene is introduced; a step of transforming by mixing PEG and the recombinant vector together with the protoplast; and a step of selecting Tolypocladium niveum using a selection marker. The transformed Tolypocladium niveum produces cyclosporine A of high concentrate.