发明名称 |
RECOMBINATION EXPRESSION VECTOR COMPRISING HMC4R OR HMC4R MUTANT PEPTIDE AND MASS-PRODUCTION METHOD OF PEPTIDE THEREOF |
摘要 |
PURPOSE: A recombinant expression vector containing hMC4R peptide or hMC4R mutation peptide is provided to easily identify three dimensional structure of the peptides. CONSTITUTION: A recombinant expression vector comprises an expression cassette in which a gene encoding hybrid partner binding protein, a gene encoding hMC4R of sequence number 1 or 2, and a gene encoding hMC4R mutant of sequence number 3 or 4 are operably bound. A method for massively producing the peptide comprises: a step of translating E.coli with a recombinant expression vector; a step of culturing the transformed E.coli in m9 medium for overpression of fusion protein; a step of dissolving the expressed fusion protein and centrifuging; a step of purifying insoluble recombinant fusion protein by Ni-affinity chromatography; a step of cleaving the insoluble recombinant fusion protein with CNBr; and a step of performing size exclusion chromatography to obtain a target peptide fragment.
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申请公布号 |
KR20100088643(A) |
申请公布日期 |
2010.08.10 |
申请号 |
KR20090007772 |
申请日期 |
2009.01.31 |
申请人 |
HANKUK UNIVERSITY OF FOREIGN STUDIES RESEARCH ANDINDUSTRY-UNIVERSITY COOPERATION FOUNDATION |
发明人 |
KIM, YONG AE;PARK, TAE JOON;CHOI, SUNG SUB;GANG, GA AE |
分类号 |
C12N15/62;C07K19/00;C12N15/12;C12N15/63 |
主分类号 |
C12N15/62 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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