| 摘要 |
Method for determination of content of hydrogen sulfide in blood serum through addition of 0.1 ml of samples of blood serum to 0.5 ml 1 % solution of zinc acetate for binding hydrogen sulfide, addition of 2.5 ml of distillated water. To the mix formed one after that adds 0.5 ml of fresh water solution of para-phenylen-diamidine hydrochloride, 0,4 ml of solution of FeClin 1,2 M HCl. Samples are incubated during 5 min. At 18-, albumen if precipitated with 1 ml of 20 % trichloroacetic acid, samples are centrifuged, with measurement of optical density of over-deposit liquid at wavelength 590 nm compared to control sample that includes 0.1 ml of 7.5 % solution of albumin. |
