| 摘要 |
The invention relates to an assay method for detecting the presence of PrPSc using at least one sequence-specific protease for a substantially complete digestion of the proteins in a sample, leaving the octarepeat region in both PrPSc and PrPC intact, leaving the protease-resistant core of PrPSc intact and remain connected to the octarepeat region, region, while digesting away at least part of the amino acid sequences in PrPC corresponding to the protease-resistant core in PrPSc. The method further comprises a step of inhibiting further digestion by the protease, a step of denaturation, and a step of detecting the presence of PrPSc using at least two binding partners, a first binding partner and a second binding partner, wherein the first binding partner specifically binds an epitope in the amino-proximal region, and the second binding partner specifically binds an epitope within a region of the protease-resistant core of PrPSc that has been cleaved away in PrPC. The invention also relates to a kit comprising reagents that can be used in the assay method.
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