METHOD FOR THE SECRETORY PRODUCTION OF HETEROLOGOUS PROTEIN IN ESCHERICHIA COLI

摘要

PURPOSE: A signal sequence peptide for improving extracellular secretion efficiency of heterologous protein in E.coli is provided to use in producing a recombinant protein. CONSTITUTION: A signal sequence peptide for improving extracellular efficiency of heterologous protein in E.coli comprises a polypeptide of sequence number 3. The polypeptide of sequence number 3 is 23 amino acid residues of beta-agarase AgaB34(Gene bank registration number EU200967; nts sequence number 1, aa sequence number 2). A gene construct for improving extracellular secretion efficiency of the heterologous protein contains a polynucleotide encoding a recombinant protein.