发明名称 |
Nukleotidsekvenser, der koder for dapC-genet, og fremgangsmåde til fremstilling af L-lysin |
摘要 |
Isolated polynucleotide comprises the dapC gene from coryneform bacteria, is new. Isolated polynucleotide (I): (i) is at least 70% identical with a sequence that encodes a protein (II) comprising a 367 amino acid (aa) sequence as given in the specification; (ii) encodes a polypeptide at least 70% identical with (II); (iii) is complementary to (i) or (ii); or (iv) contains at least 15 consecutive nucleotides from (i)-(iii). Independent claims are also included for the following: (1) a vector (III) containing (I); (2) a coryneform bacterial host cell (VI) that contains (III), or has the zwaI gene; (3) the production of L-aa, especially L-lysine, by growing bacteria in which at least the dapC gene is amplified; (4) DNA (V), from coryneform bacteria which encodes an N-succinyl-aminoketopimelate-transaminase and whose sequence is as (2) except that 209Pro is replaced by some other aa; and (5) coryneform bacteria (V) containing (V). |
申请公布号 |
DK1136559(T3) |
申请公布日期 |
2010.08.02 |
申请号 |
DK20010103850T |
申请日期 |
2001.02.16 |
申请人 |
EVONIK DEGUSSA GMBH |
发明人 |
MOECKEL, BETTINA, DR.;WEISSENBORN, ANKE;PFEFFERLE, WALTER, DR.;HARTMANN, MICHAEL;KALINOWSKI, JOERN, DR.;PUEHLER, ALFRED, PROF. |
分类号 |
C12N15/09;C12N15/31;C12N1/21;C12N9/10;C12N15/54;C12N15/63;C12N15/77;C12P13/04;C12P13/08;C12Q1/68;C12R1/13 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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