A method for the preparation of k-carrageenase

摘要

Disclosed is a method for the preparation of k-carrageenase, wherein the said method comprises the steps of: (i) growing the halotolerant marine bacterium, Pseudomonas sp. with accession number MTCC 5261 in a liquid medium to obtain culture; (ii) centrifuging the culture obtained from step (i) to obtain cell free extract as supernatant; iii) purifying the crude enzyme from supernatant obtained from step (ii) to get the pellet containing K-carrageenase; (iv) suspending the pellets obtained from step (iii); and (v) dialyzing the suspension obtained in step (iv) for removing the adhered ammonium sulphate followed by gel filtration to get desired purified K-carrageenase.